Building 4, Yuewang Wisdom Valley, 1463 Wuzhong Avenue, Wuzhong District, Suzhou, China
Cell Cytotoxicity is a critical quality attribute (CQA) for the biological activity of immune cell therapy products (e.g., NK cells, CAR-NK cells, Tm cells, CAR-T or TCR-T cells, etc.), and it is an important element of product quality research and quality control. Compared with cell cytotoxicity methods such as lactate dehydrogenase (LDH) assay, 51Cr release assay, luciferase reporter gene assay, RTCA assay, the detection of CFSE- and 7-AAD-labelled cell killing by flow cytometry is safer, more convenient, faster and more stable.
Application
·It is suitable for the evaluation of the killing function of immune cell products such as NK cells, CAR-NK cells, CAR-T cells, TCR-T cells, Tm cells, etc. on different tumour cell lines (suspended target cells).
·CMC and quality research phase: In the early development phase of cell therapy products, the cytotoxic effect is evaluated and the process is optimised.
·Release testing, stability and comparability studies phase: During the mid-term process lock phase of cell therapy products, a cell killing method for the product is established, and release testing and stability testing of the cell product are performed.
Performance Index
Data Sharing
FACS analysis of the killing rate of NK cells against CFSE-labeled K562 target cells
Cell Cytotoxicity curves of NK cells against CFSE-labelled K562 target cells
Building 4, Yuewang Wisdom Valley, 1463 Wuzhong Avenue, Wuzhong District, Suzhou, China