This kit is designed for the quantitative detection of HCP (host cell protein) content in biopharmaceuticals expressed on E.coli by using a double-antibody sandwich method.
This kit can be used to detect all components of HCP (host cell protein) in E.coli.
Specification
Assay range: 3.3-810ng/mL
Limit of quantitation: 3.3ng/mL
Precision: CV%≤10%, RE%≤±15%
Standard curve
Datasheet
Concentration of standard(ng/mL) | OD value 1 | OD value 2 | Mean value |
810 | 2.659 | 2.658 | 2.659 |
270 | 1.902 | 1.908 | 1.905 |
90 | 1.213 | 1.194 | 1.204 |
30 | 0.720 | 0.723 | 0.722 |
10 | 0.408 | 0.41 | 0.409 |
3.3 | 0.228 | 0.231 | 0.230 |
0 | 0.047 | 0.050 | 0.049 |
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