This kit determines the trace residue of Gentamicin in samples by indirect competitive ELISA. The plate is pre-coated with conjugated antigen. Gentamicin remaining in the sample and conjugated antigen pre-coated on the plate strips compete for anti-Gentamicin antibody. Add enzyme-labeled secondary antibody, and then add TMB substrate for color development. Measure the absorbance (OD value) at 450 nm/630 nm using a plate reader, and calculate the percent absorbance. The concentration of Gentamicin in the sample is negatively correlated with the percent absorbance.

Specification 

Assay range： 0.1 - 10 ng/mL

Sensitivity：0.1 ng/mL