This kit uses double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) method. Add human Granzyme B standard and test samples to the microtiter plate pre-coated with anti-human Granzyme B antibody, then add diluted biotin-labeled human Granzyme B detection antibody, finally add streptavidin-HRP to form the antibody + antigen + antibody-Biotin + SA-HRP complex, wash the plate and add TMB chromogenic solution for color development. TMB is converted from colorless to blue under the catalysis of HRP enzyme and finally to yellow under the action of stop solution. The shade of yellow is positively correlated with the amount of human Granzyme B detected in the samples.

Specification 

Assay range： 6.86 - 5000 pg/mL

Sensitivity：1.12 pg/mL