BCA Rapid Protein Quantitative Detection Kit in the BlueKit® series has the characteristics of high sensitivity, stable results, and simple operation. The principle of this kit is that Cu2+ is reduced by protein to Cu+ under alkaline conditions, and then Cu+ and BCA interact to form a purple reaction complex, showing a strong absorbance at 562 nm, and presenting a good linear relationship with protein concentration.
| Specification | 96 Test |
Assay range | 10-2000 μg/mL |
Limit of detection | 0.39 μg/mL |
| Validity period | 12 months |
| Storage conditions | RT |
| LOQ | 10 μg/mL |
| LOD | 0.39μg/mL |
| Concentration of standard (ng/mL) | OD value1 | OD value2 | Mean value |
| 0.00 | 0.1039 | 0.1039 | 0.1039 |
| 10 | 0.1196 | 0.1190 | 0.1193 |
| 25 | 0.1424 | 0.1430 | 0.1427 |
| 75 | 0.2098 | 0.2094 | 0.2096 |
| 200 | 0.3519 | 0.3540 | 0.3530 |
| 500 | 0.6408 | 0.6380 | 0.6394 |
| 1000 | 1.0423 | 1.0500 | 1.0462 |
| 2000 | 1.7229 | 1.7210 | 1.7220 |
Standard curve:
400-900-1882 
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