This kit uses double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) method. Add human TNF-α standard and test samples to the microtiter plate pre-coated with anti-human TNF-α antibody, then add diluted biotin-labeled human TNF-α detection antibody, finally add streptavidin-HRP to form the antibody + antigen + antibody-Biotin + SA-HRP complex, wash the plate and add TMB chromogenic solution for color development. TMB is converted from colorless to blue under the catalysis of HRP enzyme and finally to yellow under the action of stop solution. The shade of yellow is positively correlated with the amount of human TNF-α detected in the samples.

Specification

Assay range： 1.37 - 1000 pg/mL

Detection sensitivity：0.21 pg/mL