This product uses a double-antibody sandwich method to detect HIV-1 p24 protein in samples. A monoclonal antibody specific to HIV-1 p24 antigen is coated on a microplate, and the standard or test sample is added into the reaction well. At the same time, the anti- HIV-1 p24 secondary antibody is added and incubated at room temperature to form the antibody-antigen-secondary antibody complex. The unconjugated compounds are removed by washing and protein content in the sample is indicated by the intensity of TMB color development.
Pre-fractionation of standards are ready-to-use for convenience and reliability.
Linear range from 1.37 - 1000 ng/mL, with as few as 1-2 dilution steps, reducing operational time.
Samples and enzyme-conjugated antibodies are incubated together, with only a single cleaning step, effectively saving detection time.
Clear standards, calibrated using NIBSC/WHO (90/636) standards, ensuring reliable results.
The kit has passed verification for specificity, accuracy, precision, linearity, quantitation limit, detection limit, durability, and stability in accordance with pharmacopeia standards. Production release is traceable, ensuring quality control.
Over 100 customers have used the product, with 10 IND submissions approved using it.
Optimization of the detection steps during product design minimizes detection bias caused by operator variability.
Short detection time, with results available in 1 hour, reducing systematic detection deviations.
Error proofing design: All components are color-coded to avoid confusion during use.
Dedicated product developers available for guidance, reducing communication time and costs.
24-hour service response mechanism to resolve issues immediately.
Exclusive shipping methods to ensure products are delivered directly to users as needed.
| Specification | 96 Test |
Assay range | 1.37-1000ng/mL |
Sensitivity | 0.35ng/mL |
Precision | CV%≤10%, RE%≤±15% |
| Validity period | 12 months |
| Storage conditions | 4℃ |
| LOQ | 1.37ng/mL |
| LOD | 1 ng/mL |
Lentivirus Titer p24 Rapid ELISA Detection Kit is used for p24 protein and titer detection of HIV-1 lentiviral samples. It is rapid (pre-fractionation of standards), convenient (single incubation and single wash), and stable. Experimenter can be used to complete the detection of the p24 protein of HIV-1 lentiviral samples in less than one hour.
| Batch No. | Hillgene(ng/mL) | Brand T (ng/mL) | CV |
| A | 6.58E+03 | 5.19E+03 | 17% |
| B | 4.88E+03 | 3.35E+03 | 26% |
| C | 5.15E+03 | 3.42E+03 | 29% |
| D | 5.53E+03 | 4.94E+03 | 8% |
| E | 3.85E+03 | 3.33E+03 | 10% |
| F | 3.77E+03 | 3.26E+03 | 10% |
Figure 1： Standard Curve （Four-ParameterFit）
Figure 2： Comparison of Detection Times with Competitor p24 ELISA Products
Figure 3: Comparison of Detection Range with Competitor p24 Products
Figure 4: Comparison of Competitor Detection Data
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info@hillgene.com 
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