This kit is designed for the quantitative detection of residual E.coli total RNA in various biological products to improve control quality of nucleic acid.
This kit adopts the principle of the RT-PCR fluorescent probe, combining reverse transcription PCR . technology and fluorescent probe method, to realize one-step quantitative detection.
Limit of Quantitation (LOQ) of 2.00 fg/μL, much more sensitive RT-qPCR than HPLC
Target sequence: E.coli 23S rRNA
Reference standards traceable
Suitable for most E. coli: DH5α, BL21, Top10
Compatible with most qPCR instruments, e.g., ABI 7500, C480, etc.
Preprocessing kit for plasmid samples also available
| Specification | 100 Reactions |
Assay range | 2.00 ~2.00×104 fg/μL |
Limit of quantitation | 2.00 fg/μL |
Limit of detection | 0.50 fg/μL |
Precision | CV%≤15% |
| Validity period | 18 months |
| Storage conditions | -20℃ |
| LOQ | 2 fg/μL |
| LOD | 0.2 fg/μL |
| IND Filing | OK |
| FDA Filing | OK |
Concentration | Log10 | Ct Value(1) | Ct Value(2) | Ct Value(3) | Ct Mean Value | Recovery rate |
2.00E+04 | 4.30 | 15.30 | 15.23 | 15.21 | 15.24 | 0.30% |
2.00E+03 | 3.30 | 18.39 | 18.28 | 18.18 | 18.28 | 0.56% |
2.00E+02 | 2.30 | 21.86 | 21.82 | 21.78 | 21.82 | 0.18% |
2.00E+01 | 1.30 | 25.22 | 25.22 | 25.09 | 25.18 | 0.31% |
2.00E+00 | 0.30 | 28.55 | 28.58 | 28.43 | 28.52 | 0.29% |
Amplification efficiency 99.1% | 99.1% | |||||
Standard curve:
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info@hillgene.com 
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