This kit is designed for the quantitative detection of residual T7 RNA Polymerase content added in RNA pharmaceuticals processes by using a double-antibody sandwich method .
Specification
Assay range:4-256 ng/mL
Limit of quantitation:4 ng/mL
Limit of detection:2 ng/mL
Precision: CV%≤10%, RE%≤±15%
Standard curve
Datasheet
Concentration of standard(ng/mL) | OD value 1 | OD value 2 | Mean value |
256 | 3.109 | 3.183 | 3.146 |
128 | 2.281 | 2.213 | 2.247 |
64 | 1.411 | 1.394 | 1.4025 |
32 | 0.787 | 0.845 | 0.816 |
16 | 0.463 | 0.447 | 0.455 |
8 | 0.299 | 0.307 | 0.303 |
4 | 0.235 | 0.235 | 0.235 |
0 | 0.141 | 0.133 | 0.137 |
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