This kit uses double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) method. Add human TGF-β1 standard and test samples to the microtiter plate pre-coated with anti-human TGF-β1 antibody, then add diluted biotin-labeled human TGF-β1 detection antibody, finally add streptavidin-HRP to form the antibody + antigen + antibody-Biotin + SA-HRP complex, wash the plate and add TMB chromogenic solution for color development. TMB is converted from colorless to blue under the catalysis of HRP enzyme and finally to yellow under the action of stop solution. The shade of yellow is positively correlated with the amount of human TGF-β1 detected in the samples.
| Specification | 96 Test |
Assay range | 156.25 - 10000 pg/mL |
Detection sensitivity | 33.32 pg/mL |
| Validity period | 12 months |
| Storage conditions | 4℃ |
| LOQ | 156.25pg/mL |
| LOD | 33.32pg/mL |
| Concentration of standard (pg/mL) | OD value1 | OD value2 | Mean value |
| 10000 | 3.3531 | 3.2747 | 3.3139 |
| 5000 | 1.9813 | 2.0695 | 2.0254 |
| 2500 | 1.1123 | 1.1675 | 1.1399 |
| 1250 | 0.5891 | 0.6252 | 0.6072 |
| 625 | 0.3551 | 0.3739 | 0.3645 |
| 312.5 | 0.2321 | 0.2237 | 0.2279 |
| 156.25 | 0.1488 | 0.1692 | 0.159 |
| 0.00 | 0.0886 | 0.0786 | 0.0836 |
Standard curve:
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