This kit is designed for the quantitative detection of residual CHO DNA content in intermediates, semi-finished products and finished products of various biological products.
This kit adopts the principle of the Taqman probe to quantitatively detect CHO residual DNA in samples. The kit is a rapid, specific and reliable device, with the minimum detection limit reaching fg level.
Specification | 100 Reactions |
Assay range | 3.00~3.00x105fg/μL |
Limit of quantitation | 3fg/μL |
Precision | CV%≤15% |
Validity period | 12 months |
Storage conditions | -20℃ |
LOQ | 3fg/μL |
LOD | NA |
Concentration (fg/μl) | Log10 Concentration | Ct Value(1) | Ct Value(2) | Ct Value(3) | Ct Mean Value | CV |
3.00E+05 | 5.48 | 15.94 | 16.08 | 16.12 | 16.05 | 0.59% |
3.00E+04 | 4.48 | 19.10 | 19.16 | 19.17 | 19.14 | 0.18% |
3.00E+03 | 3.48 | 22.72 | 22.89 | 22.95 | 22.86 | 0.51% |
3.00E+02 | 2.48 | 26.26 | 26.38 | 26.30 | 26.31 | 0.23% |
3.00E+01 | 1.48 | 29.61 | 29.70 | 29.79 | 29.70 | 0.30% |
3.00E+00 | 0.48 | 33.88 | 33.28 | 33.43 | 33.53 | 0.93% |
Amplification efficiency | 93.00% |
Standard curve: