Building 4, Yuewang Wisdom Valley, 1463 Wuzhong Avenue, Wuzhong District, Suzhou, China
This kit is designed for the quantitative detection of CAR gene copy number in the genome of CAR-T/TCR-T cells prepared by using HIV-1 lentiviral vector technology.
This kit adopts the fluorescent probe method and multiplex PCR method to detect the DNA sequence related to integration or expression function on the transfer plasmid and the reference gene (RFG) in human cells, and the CAR gene copy number/cell in the sample can be calculated.
The kit is a rapid, specific and reliable device.
Specification
Assay range: 3.00×101~3.00×106copies/μL
Limit of quantitation: 30 copies/μL
Limit of detection: 15 copies/μL
Precision: CV%≤15%
Standard curve
Datesheet
| Copies/μl | Copies Log Value | Target Gene Copies Standard Curve | RFG Gene Copies Standard Curve | ||||||||
| Ct Value（1） | Ct Value（2） | CtValue（3） | Ct Mean Value | Recovery Rate | Ct Value（1） | Ct Value（2） | Ct Value（3） | Ct Mean Value | Recovery Rate | ||
| 3.00E+06 | 6.48 | 16.14 | 16.29 | 16.21 | 16.21 | 93.33% | 15.39 | 15.48 | 15.42 | 15.43 | 97.93% |
| 3.00E+05 | 5.48 | 19.27 | 19.61 | 19.43 | 19.43 | 109.50% | 18.67 | 18.79 | 18.71 | 18.72 | 101.97% |
| 3.00E+04 | 4.48 | 23.00 | 23.04 | 23.01 | 23.01 | 101.34% | 22.09 | 22.12 | 22.03 | 22.08 | 101.49% |
| 3.00E+03 | 3.48 | 26.42 | 26.53 | 26.54 | 26.54 | 100.21% | 25.43 | 25.46 | 25.45 | 25.45 | 100.45% |
| 3.00E+02 | 2.48 | 30.10 | 30.00 | 30.06 | 30.06 | 94.34% | 28.85 | 28.80 | 28.88 | 28.84 | 97.50% |
| 3.00E+01 | 1.48 | 33.41 | 33.50 | 33.27 | 33.27 | 102.29% | 32.25 | 32.18 | 32.00 | 32.14 | 100.80% |
| Amplification Efficiency | 94.4% | Amplification Efficiency | 98.82% | ||||||||
Building 4, Yuewang Wisdom Valley, 1463 Wuzhong Avenue, Wuzhong District, Suzhou, China