This kit is designed for the quantitative detection of residual Dnase I content added in RNA pharmaceuticals processes by using a double-antibody sandwich method.
Assay range | 1-64 ng/mL |
Limit of quantitation | 1 ng/mL |
Limit of detection | 0.5 ng/mL |
Precision | CV%≤10%, RE%≤±15% |
| Concentration of standard (ng/mL) | OD value 1 | OD value 2 | Mean value |
| 64 | 2.373 | 2.347 | 2.360 |
| 32 | 1.520 | 1.546 | 1.533 |
| 16 | 0.933 | 0.934 | 0.9335 |
| 8 | 0.518 | 0.536 | 0.527 |
| 4 | 0.259 | 0.291 | 0.275 |
| 2 | 0.166 | 0.168 | 0.167 |
| 1 | 0.115 | 0.109 | 0.112 |
| 0 | 0.057 | 0.049 | 0.053 |
Standard curve:
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