This kit is designed for the quantitative detection of the 293T cell DNA in intermediate, semi-finished and finished products of various biological products.
This kit adopts the principle of the Taqman probe to quantitatively detect 293T Cell Residual DNA in samples. The kit is a rapid, specific and reliable device, with the minimum detection limit reaching fg level.
| Specification | 100 Reactions |
Assay range | 3.00×10¹~3.00×10⁵ fg/μL |
Limit of quantitation | 3.00×10¹ fg/μL |
Precision | CV%≤15% |
| Validity period | 18 months |
| Storage conditions | -20℃ |
| LOQ | 30 fg/μL |
| LOD | 3 fg/μL |
| Concentration (fg/μl) | Log Concentration | Ct Value(1) | Ct Value(2) | Ct Value(3) | Ct Mean Value | Recovery rate |
| 3.00E+05 | 5.48 | 23.64 | 23.71 | 23.59 | 23.65 | 92.00% |
| 3.00E+04 | 4.48 | 26.79 | 26.85 | 26.78 | 26.81 | 97.00% |
| 3.00E+03 | 3.48 | 30.08 | 30.11 | 30.03 | 30.07 | 101.00% |
| 3.00E+02 | 2.48 | 33.89 | 33.73 | 33.76 | 33.79 | 99.00% |
| 3.00E+01 | 1.48 | 36.03 | 36.52 | 36.39 | 36.31 | 91.00% |
| Amplification efficiency | 97.90% | |||||
Standard curve:
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