This kit is designed for the quantitative detection of residual Human total RNA in various biological products to improve control quality of nucleic acid.
This kit adopts the principle of the RT-PCR fluorescent probe, combining reverse transcription PCR.technology and fluorescent probe method, to realize one-step quantitative detection.
| Specification | 100 Reactions |
Assay range | 2.00~2.00×104 fg/μL |
Limit of quantitation | 2.00 fg/μL |
Limit of detection | 0.50 fg/μL |
Precision | CV%≤15% |
| Validity period | 18 months |
| Storage conditions | -20℃ |
| LOQ | 2 fg/μL |
| LOD | 2 fg/μL |
| Concentration (fg/μl) | Log Concentration | Ct Value(1) | Ct Value(2) | Ct Value(3) | Ct Mean Value | Recovery rate |
| 2.00E+04 | 4.3 | 16.45 | 16.37 | 16.36 | 16.39 | 0.30% |
| 2.00E+03 | 3.3 | 19.41 | 19.51 | 19.44 | 19.46 | 0.26% |
| 2.00E+02 | 2.3 | 23.03 | 22.83 | 22.98 | 22.95 | 0.45% |
| 2.00E+01 | 1.3 | 26.31 | 26.33 | 26.26 | 26.3 | 0.12% |
| 2.00E+00 | 0.3 | 29.53 | 29.48 | 29.42 | 29.48 | 0.19% |
| Amplification efficiency | 100.90% | |||||
Standard curve:
400-900-1882 
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