This dsRNA ELISA detection kits of BlueKit®series use a double antibody sandwich method to quantitatively detect the content of double stranded RNA (dsRNA) in samples. The detected dsRNA is 60 bp or more in length, and is not related to its nucleic acid sequence.
| Standard type | Assay range (pg/μL) | Limit of quantitation (pg/μL) | Limit of detection (pg/μL) | Recovery rate | CV% |
| No modification | 0.0156-0.5 | 0.0156 | 0.001 | 80-120% | ≤10% |
| N1-Me-pUTP modified | 0.0312-1 | 0.0312 | 0.001 | 80-120% | ≤10% |
| pUTP modified | 0.0156-0.5 | 0.0156 | 0.001 | 80-120% | ≤10% |
| 5-OMe-UTP modified | 0.0625-1 | 0.0625 | 0.01 | 80-120% | ≤10% |
| Specification | 96 Test |
| Validity period | 12 months |
| Storage conditions | 4℃ |
| LOQ | pUT：0.0156pg/μL N1-Me-pUTP：0.0312pg/μL 5-OMe-UTP：0.0625pg/μL |
| LOD | pUTP：0.001pg/μL N1-Me-pUTP：0.001pg/μL 5-0Me-UTP：0.01pg/μLL |
| Concentration of standard (pg/μL) | OD value1 | OD value2 | Mean value |
| 2 | 2.8412 | 2.7362 | 2.7887 |
| 1 | 1.8725 | 1.9135 | 1.8930 |
| 0.5 | 1.0863 | 1.1207 | 1.1035 |
| 0.25 | 0.623 | 0.6055 | 0.6143 |
| 0.125 | 0.3388 | 0.3292 | 0.3340 |
| 0.0625 | 0.1947 | 0.1885 | 0.1916 |
| 0.0312 | 0.1192 | 0.1247 | 0.1220 |
| 0 | 0.0567 | 0.0518 | 0.0543 |
Standard curve:
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