Plasmid Residual DNA Detection Kit targets the sequences of kanamycin resistance genes commonly found in plasmids and is able to quantitatively detect all plasmid DNA residues with kanamycin resistance. This kit is based on the principle of TaqMan fluorescence probe, with potent specificity, high sensitivity and reliable performance, and is used as a kit specially designed for the detection of plasmid DNA residues in intermediates, bulk products and final products of various biological products
KanR Plasmid DNA Kit (40 copies/μL LOQ)
Detects trace residuals in viral vectors - 10x more sensitive than gel electrophoresis.
Universal Kanamycin Resistance Detection
Covers all common KanR plasmids (pUC, pBR322, etc.).
GMP-Ready qPCR (CV≤15%)
Pre-validated per ICH Q6B for gene therapy QC.
Assay range | 4×101~4×106copies/μL |
Limit of quantitation | 4×101copies/μL |
Precision | CV%≤15% |
| Concentration (copies/μL) | Log10 Concentration | Ct Value(1) | Ct Value(2) | Ct Value(3) | Ct Mean Value | CV |
| 4.00E+06 | 6.60 | 16.09 | 15.84 | 15.93 | 15.95 | 0.78% |
| 4.00E+05 | 5.60 | 18.88 | 18.83 | 18.92 | 18.87 | 0.25% |
| 4.00E+04 | 4.60 | 22.31 | 22.50 | 22.43 | 22.42 | 0.43% |
| 4.00E+03 | 3.60 | 25.69 | 25.49 | 25.58 | 25.59 | 0.38% |
| 4.00E+02 | 2.60 | 28.69 | 28.69 | 28.47 | 28.62 | 0.45% |
| 4.00E+01 | 1.60 | 32.11 | 32.01 | 32.08 | 32.07 | 0.16% |
| Amplification efficiency | 103.80% | |||||
Standard curve:
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