This kit is designed for the quantitative detection of residual plasmid DNA in intermediates, semifinished products and finished products of various biological products. Plasmid DNA content in samples (e.g., lentivirus, adenovirus) is detected by analyzing the consensus sequence.
This kit uses TaqMan fluorescence probe principle, with strong specificity, high sensitivity and reliable performance.
Assay range | 4×101~4×106copies/μL |
Limit of quantitation | 4×101copies/μL |
Precision | CV%≤15% |
| Concentration (copies/μl) | Log10 Concentration | Ct Value(1) | Ct Value(2) | Ct Value(3) | Ct Mean Value | CV |
| 4.00E+06 | 6.60 | 16.76 | 17.14 | 17.07 | 16.99 | 1.18% |
| 4.00E+05 | 5.60 | 19.93 | 20.32 | 20.20 | 20.15 | 1.01% |
| 4.00E+04 | 4.60 | 23.59 | 23.99 | 23.85 | 23.81 | 0.85% |
| 4.00E+03 | 3.60 | 26.90 | 27.34 | 27.37 | 27.20 | 0.97% |
| 4.00E+02 | 2.60 | 30.09 | 30.54 | 30.41 | 30.35 | 0.76% |
| 4.00E+01 | 1.60 | 33.27 | 33.20 | 33.57 | 33.35 | 0.60% |
| Amplification efficiency | 100.6% | |||||
Standard curve:
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