This kit uses double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) method. Add PPase standard and test samples to the microtiter plate precoated with anti-PPase antibody, then add diluted biotin-labeled PPase detection antibody, finally add streptavidin-HRP to form the antibody + antigen + antibody-Biotin + SA-HRP complex, wash the plate and add TMB chromogenic solution for color development. TMB is converted from colorless to blue under the catalysis of HRP enzyme and finally to yellow under the action of stop solution. The shade of yellow is positively correlated with the amount of PPase detected in the sample.
Biotin-SA-HRP amplification boosts signal-to-noise vs. conventional ELISAs
Broad 64x dynamic range (0.25–16 ng/mL) – no dilutions needed
21 CFR Part 11-ready data templates for audits
Sample/standard preparation, sample loading, incubation and plate washing
Preparation of detection antibodies and plate washing
Preparation of enzyme-conjugated antibodies and plate washing
Color development and reaction termination
Reading
| Specification | 96 Test |
Assay range | 0.25 - 16 ng/mL |
Limit of quantitation | 0.25 ng/mL |
| Validity period | 12 months |
| Storage conditions | 4℃ |
| LOQ | 0.25ng/mL |
| LOD | 0.25ng/mL |
| Concentration of standard (ng/μL) | OD value1 | OD value2 | Mean value |
| 16 | 2.698 | 2.612 | 2.655 |
| 8 | 1.952 | 1.864 | 1.908 |
| 4 | 1.202 | 1.265 | 1.234 |
| 2 | 0.699 | 0.729 | 0.714 |
| 1 | 0.439 | 0.454 | 0.447 |
| 0.5 | 0.265 | 0.281 | 0.273 |
| 0.25 | 0.192 | 0.183 | 0.188 |
| 0 | 0.094 | 0.091 | 0.093 |
Standard curve:
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