Compared with the three-plasmid vector system, the four-plasmid vector system greatly reduces the possibility of RCL production and increases the safety of the vector system. The third generation lentiviral packaging platform contains four plasmids in the plasmid system, including a shuttle plasmid containing exogenous target genes and three helper plasmids which include two packaging plasmids and one envelope plasmid, respectively.
The premade plasmid products (HiPlas ™ LentiHelper Plasmids for T) on the platform plasmid system for lentiviral packaging of CAR-T, TCR-T and other lentivirus provided by Jiangsu Hillgene can meet the needs of customers at various stages of pre-clinical research, IIT, IND application, clinical trials and commercialization.
Assay range | 3.00×101~3.00×106copies/μL |
Limit of quantitation | 30 copies/μL |
Limit of detection | 15 copies/μL |
Precision | CV%≤15% |
| Copies/μl | Copies Log Value | Target Gene Copies Standard Curve | RFG Gene Copies Standard Curve | ||||||||
| Ct Value（1） | Ct Value（2） | CtValue（3） | Ct Mean Value | Recovery Rate | Ct Value（1） | Ct Value（2） | Ct Value（3） | Ct Mean Value | Recovery Rate | ||
| 3.00E+06 | 6.48 | 16.14 | 16.29 | 16.21 | 16.21 | 93.33% | 15.39 | 15.48 | 15.42 | 15.43 | 97.93% |
| 3.00E+05 | 5.48 | 19.27 | 19.61 | 19.43 | 19.43 | 109.50% | 18.67 | 18.79 | 18.71 | 18.72 | 101.97% |
| 3.00E+04 | 4.48 | 23.00 | 23.04 | 23.01 | 23.01 | 101.34% | 22.09 | 22.12 | 22.03 | 22.08 | 101.49% |
| 3.00E+03 | 3.48 | 26.42 | 26.53 | 26.54 | 26.54 | 100.21% | 25.43 | 25.46 | 25.45 | 25.45 | 100.45% |
| 3.00E+02 | 2.48 | 30.10 | 30.00 | 30.06 | 30.06 | 94.34% | 28.85 | 28.80 | 28.88 | 28.84 | 97.50% |
| 3.00E+01 | 1.48 | 33.41 | 33.50 | 33.27 | 33.27 | 102.29% | 32.25 | 32.18 | 32.00 | 32.14 | 100.80% |
| Amplification Efficiency | 94.4% | Amplification Efficiency | 98.82% | ||||||||
Standard curve:
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