Product Name: Hela Cell Residual DNA Detection Kit (qPCR)
This kit is designed for the quantitative detection of the Hela Cell DNA in intermediate, semi-finished and finished products of various biological products.
This kit adopts the principle of the Taqman probe to quantitatively detect Hela Cell residual DNA in samples. The kit is a rapid, specific and reliable device, with the minimum detection limit reaching fg level.
HeLa-specific probes - No cross-reactivity with other human cell lines
Ready-to-use mastermix - Saves 1.5 hours vs. competitor kits
Includes NIST-traceable standards - Meets FDA "≤10 ng/dose" requirements
| Specification | 100 Reactions |
Assay range | 3.00×10¹~3.00×10⁵ fg/μL |
Limit of quantitation | 3.00×10¹ fg/μL |
Precision | CV%≤15% |
| Validity period | 18 months |
| Storage conditions | -20℃ |
| LOQ | 30 fg/μL |
| LOD | 3 fg/μL |
| Concentration (fg/μL) | Log Concentration | Ct Value(1) | Ct Value(2) | Ct Value(3) | Ct Mean Value | Recovery rate |
| 3.00E+05 | 5.48 | 22.69 | 22.53 | 22.58 | 22.6 | 0.36% |
| 3.00E+04 | 4.48 | 25.73 | 25.61 | 25.52 | 25.62 | 0.42% |
| 3.00E+03 | 3.48 | 29.06 | 29.02 | 28.98 | 29.02 | 0.14% |
| 3.00E+02 | 2.48 | 32.35 | 32.71 | 32.63 | 32.56 | 0.59% |
| 3.00E+01 | 1.48 | 36.11 | 35.68 | 35.46 | 35.75 | 0.93% |
| Amplification efficiency | 99.86% | |||||
Standard curve:
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