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Medical Consumables and Lab Consumables OEM Manufacturer

Host Cell DNA Residual Detection Kit for HEK293(qPCR)

Product Name: HEK293 Cell Residual DNA Detection Kit (qPCR)

This kit is designed for the quantitative detection of the HEK293  Cell DNA in intermediate, semi-finished and finished products of various biological products.


This kit adopts the principle of the Taqman probe to quantitatively detect HEK293 Cell residual DNA in samples. The kit is a rapid, specific and reliable device, with the minimum detection limit reaching fg level.

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SKU: HG-HD003
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Features of Host Cell DNA Residual Detection Kit for HEK293(qPCR)

High Sensitivity Detection

fg-level sensitivity (30-300,000 fg/μL)

TaqMan probe technology

4-log dynamic range


Ready-to-Use Convenience

Pre-mixed master mix

18-month stability (-20°C)

Universal ROX options


Compliance-Ready QC

Full validation data (R²≥0.98)

50-150% spike recovery

Built-in controls (NTC/NCS)


Specification of HEK293 Cell Residual DNA Detection Kit (qPCR)


Specification100 Reactions

Assay range

3.00×10¹~3.00×10⁵ fg/μL

Limit of quantitation

3.00×10¹ fg/μL

Precision

CV%≤15%

Validity period18 months
Storage conditions-20℃
LOQ30 fg/μL
LOD3 fg/μL

Data of HEK293 Cell Residual DNA Detection Kit (qPCR)

Concentration(fg/μl)Log ConcentrationCt Value(1)Ct Value(2)Ct Value(3)Ct Mean ValueRecovery rate
3.00E+055.4823.5223.3723.3923.4388%
3.00E+044.4826.6426.5526.4526.54111%
3.00E+033.4830.1630.0730.0630.09105%
3.00E+022.4833.7533.3333.5233.53107%
3.00E+011.4838.336.4936.9037.2690%
Amplification efficiency94.34%


Standard curve:

Data of HEK293 Cell Residual DNA Detection Kit (qPCR)

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