Product Name: HEK293 Cell Residual DNA Detection Kit (qPCR)
This kit is designed for the quantitative detection of the HEK293 Cell DNA in intermediate, semi-finished and finished products of various biological products.
This kit adopts the principle of the Taqman probe to quantitatively detect HEK293 Cell residual DNA in samples. The kit is a rapid, specific and reliable device, with the minimum detection limit reaching fg level.
fg-level sensitivity (30-300,000 fg/μL)
TaqMan probe technology
4-log dynamic range
Pre-mixed master mix
18-month stability (-20°C)
Universal ROX options
Full validation data (R²≥0.98)
50-150% spike recovery
Built-in controls (NTC/NCS)
| Specification | 100 Reactions |
Assay range | 3.00×10¹~3.00×10⁵ fg/μL |
Limit of quantitation | 3.00×10¹ fg/μL |
Precision | CV%≤15% |
| Validity period | 18 months |
| Storage conditions | -20℃ |
| LOQ | 30 fg/μL |
| LOD | 3 fg/μL |
| Concentration(fg/μl) | Log Concentration | Ct Value(1) | Ct Value(2) | Ct Value(3) | Ct Mean Value | Recovery rate |
| 3.00E+05 | 5.48 | 23.52 | 23.37 | 23.39 | 23.43 | 88% |
| 3.00E+04 | 4.48 | 26.64 | 26.55 | 26.45 | 26.54 | 111% |
| 3.00E+03 | 3.48 | 30.16 | 30.07 | 30.06 | 30.09 | 105% |
| 3.00E+02 | 2.48 | 33.75 | 33.33 | 33.52 | 33.53 | 107% |
| 3.00E+01 | 1.48 | 38.3 | 36.49 | 36.90 | 37.26 | 90% |
| Amplification efficiency | 94.34% | |||||
Standard curve:
