Hillgene dsRNA ELISA detection kits of BlueKit®series use a double antibody sandwich method to quantitatively detect the content of double-stranded RNA (dsRNA) in samples. Our dsRNA detection kit identifies dsRNA molecules of 60 bp or more in length, with detection being independent of the nucleic acid sequence. This specific capability makes the Hillgene dsRNA detection kit an essential tool for quality control in mRNA vaccine and therapeutics manufacturing.
| Standard type | Assay range (pg/μL) | Limit of quantitation (pg/μL) | Limit of detection (pg/μL) | Recovery rate | CV% |
| No modification | 0.0156-0.5 | 0.0156 | 0.001 | 80-120% | ≤10% |
| N1-Me-pUTP modified | 0.0312-1 | 0.0312 | 0.001 | 80-120% | ≤10% |
| pUTP modified | 0.0156-0.5 | 0.0156 | 0.001 | 80-120% | ≤10% |
| 5-OMe-UTP modified | 0.0625-1 | 0.0625 | 0.01 | 80-120% | ≤10% |
| Specification | 96 Test |
| Validity period | 12 months |
| Storage conditions | 4℃ |
| LOQ | pUT：0.0156pg/μL N1-Me-pUTP：0.0312pg/μL 5-OMe-UTP：0.0625pg/μL |
| LOD | pUTP：0.001pg/μL N1-Me-pUTP：0.001pg/μL 5-0Me-UTP：0.01pg/μLL |
| Concentration of the standard (pg/μL) | OD value1 | OD value2 | Mean value |
| 2 | 2.8412 | 2.7362 | 2.7887 |
| 1 | 1.8725 | 1.9135 | 1.8930 |
| 0.5 | 1.0863 | 1.1207 | 1.1035 |
| 0.25 | 0.623 | 0.6055 | 0.6143 |
| 0.125 | 0.3388 | 0.3292 | 0.3340 |
| 0.0625 | 0.1947 | 0.1885 | 0.1916 |
| 0.0312 | 0.1192 | 0.1247 | 0.1220 |
| 0 | 0.0567 | 0.0518 | 0.0543 |
Standard curve of dsRNA ELISA kit:
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