CD19 CAR T cell preparation kit is composed of three parts: T cell activation and expansion unit, T cell purification unit and CD19 CAR gene transduction unit. The T cell activation and expansion unit consists of T cell basal medium and T cell activation reagent, which provides nutrients for T cell culture, and the T cell activation reagent provides T cell activation signals for T cell activation; the T cell purification unit consists of T cell sorting beads and columns, which is capable of obtaining high purity T cells from single blood collection or PBMC; The CD19 CAR gene transduction unit contains CD19 CAR T spot lentivirus and T cell transduction enhancer (Viral E-hancer B), which enables efficient integration of CD19 CAR sequence. This kit is easy to operate, and prepares CAR T cells with high expansion multiplicity and cell viability, CAR gene transduction efficiency and T cell purity.
| Performance Program | Indicator results |
| T cell purity | >95% |
| CAR+% | > 20% |
| Cell viability | > 90% |
Applicable to the preparation of CD19 CAR-T cells of peripheral blood and cord blood origin.
Process development stage: shorten the time and cost of early process development of cell therapy products, and better help customers advance the development of CD19 CAR T cell therapy productsd.
Purity of T cells before and after sorting:

Cell viability and expansion:

T cell transduction efficiency:
