Kanamycin ELISA Detection Kit-2G

This kit uses indirect competitive ELISA to detect trace residues of kanamycin in samples.The microplate is pre-coated with a conjugate antigen. Kanamycin residues in the sample compete with the pre-coated conjugate antigen on the microplate for binding to anti-kanamycin antibodies. After adding the enzyme-labeled secondary antibody, the reaction is visualized by adding TMB substrate. The absorbance (OD value) is measured at 450 nm/630 nm using a microplate reader, and the percentage absorbance is calculated. The kanamycin concentration in the sample is inversely proportional to the percentage absorbance.