Hillgene's NK and TIL Cell Expansion Reagent (Feeder cell) is an irradiated and inactivated engineered K562 cells expressing IL-21 and other cytokines, which, together with the mainstream immune cell culture medium, can activate and expand NK cells and tumor-infiltrating TIL cells derived from umbilical cord blood and peripheral blood mononuclear cells cultured in vitro, and the purity of the obtained NK and TIL cells is high.
Currently, several NK cells and TILs cell products produced using trophoblast cells have been approved for clinical use or marketed globally, and studies have shown that genetically modified K562 trophoblast cells can achieve more than 10,000-fold expansion of NK or CAR-NK cells in 15 days, which can meet the demand for their large-volume preparation.
Pre-irradiated, multi-cytokine enhanced for superior NK/TIL expansion.
Validated for both cord blood and PBMC sources - CAR-ready.
Pre-qualified for IND submissions with full characterization data.
D0: Plasma and PBMC isolation, inoculation and activation
D4: Observe and medium exchange
D6: Observe and medium infusion
D8: Observe and medium infusion, re-activation
D10: Observe and medium exchange
D12: Observe and medium infusion
D14: Harvest
| Performance Program | Indicator results |
| NK/CAR-NK cell expansion | Over 5000 times (14d) |
| NK/CAR-NK cell viability | > 90% |
| NK/CAR-NK Cell purity | > 95% |
Fold expansion and viability of different Donor-derived NK cells:
Purity of different Donor-derived NK cells:
86 198 5711 6962 
info@hillgene.com 
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