The E1A residual DNA detection kit can rapidly and specifically detect E1A DNA residues derived from host cells (such as HEK293T cells) in biological products.
This kit quantitatively detects human residual DNA in samples based on Taqman probe principle. The kit is a rapid, specific and reliable device, with the minimum detection limit reaching 40 copies/μL
| Specification | 100 Reactions |
Assay range | 4×101 ~4×106copies/μL |
Limit of quantitation | 4×101copies/μL |
Precision | CV%≤15% |
| Validity period | 18 months |
| Storage conditions | -20℃ |
| LOQ | 40 copies fg/μL |
| LOD | 5 copies fg/μL |
| Concentration (copies/μL) | Log10 Concentration | Ct Value(1) | Ct Value(2) | Ct Value(3) | Ct Mean Value | CV |
| 4.00E+06 | 6.60 | 11.64 | 11.69 | 11.42 | 11.58 | 1.27% |
| 4.00E+05 | 5.60 | 14.83 | 14.84 | 14.80 | 14.82 | 0.13% |
| 4.00E+04 | 4.60 | 18.24 | 18.02 | 18.28 | 18.18 | 0.77% |
| 4.00E+03 | 3.60 | 21.79 | 21.65 | 21.92 | 21.78 | 0.62% |
| 4.00E+02 | 2.60 | 25.02 | 25.07 | 25.05 | 25.05 | 0.09% |
| 4.00E+01 | 1.60 | 28.50 | 28.38 | 28.66 | 28.51 | 0.50% |
| Amplification efficiency | 96.94% | |||||
Standard curve:
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