Host Cell DNA DNA Fragment Residual Detection Kit for Escherichia coli（qPCR）

Name: Host Cell DNA DNA Fragment Residual Detection Kit for Escherichia coli（qPCR）
Brand: Hillgene Biopharma Co., Ltd.
Availability: InStock

Product Name:E.coli Residual DNA Fragment Analysis Detection Kit (qPCR)

Based on fluorescent probe method, this kit quantitatively detects fragments of E.coli residual DNA with sizes of 62bp, 141bp, 284bp, and 517bp, respectively, covering the possible functional gene sizes to the greatest extent, with the sensitivity up to 3fg/μL, and can accurately quantify the residual amount and distribution of each fragment of E. coli DNA.

The kit is equipped with the sample preprocessing kit of our company (Cat. No.: HG-CL100) for sample preprocessing.

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SKU: HG-EF001

Features of Host Cell DNA DNA Fragment Residual Detection Kit for Escherichia coli（qPCR）

General Workflow

Service Details

Service Features

Case Studies

Specification of E.coli Residual DNA Fragment Analysis Detection Kit (qPCR)

Specification	400 Reactions
Assay range	3.00×10¹~3.00×10⁵ fg/μL
Limit of quantitation	3.00×10¹ fg/μL
Precision	CV%≤15%
Validity period	18 months
Storage conditions	-20℃
LOQ	3 fg/μL
LOD	3 fg/μL
IND Filing	NA
FDA Filing	NA

Residual DNA fragment(≥62bp) Detection

Standard		Target gene copy number standard curve (FAM)
Concentration (fg/μL) Log10 Concentration		Ct Value 1	Ct Value 2	Ct Value 3	CV%
3.00E+05	5.48	15.809	15.717	15.76	0.41%
3.00E+04	4.48	19.311	19.166	19.24	0.54%
3.00E+03	3.48	22.547	22.666	22.61	0.37%
3.00E+02	2.48	26.056	26.120	26.09	0.17%
3.00E+01	1.48	29.472	29.639	29.56	0.40%
3.00	0.48	32.509	32.810	32.66	0.65%
Amplification efficiency				97.43%

Residual DNA fragment(≥141bp) Detection

Standard		Target gene copy number standard curve (FAM)
Concentration (fg/uL) Log10 Concentration		Ct Value 1	Ct Value 2	Ct Value 3	CV%
3.00E+05	5.48	17.282	17.331	17.31	0.20%
3.00E+04	4.48	20.748	20.806	20.78	0.20%
3.00E+03	3.48	24.205	24.215	24.21	0.03%
3.00E+02	2.48	27.496	27.627	27.56	0.34%
3.00E+01	1.48	30.813	31.106	30.96	0.67%
3.00	0.48	34.156	34.068	34.11	0.18%
Amplification efficiency				97.71%

Residual DNA fragment(≥284bp) Detection

Standard		Target gene copy number standard curve (FAM)
Concentration (fg/uL) Log10 Concentration		Ct Value 1	Ct Value 2	Ct Value 3	CV%
3.00E+05	5.48	17.707	17.800	17.75	0.37%
3.00E+04	4.48	21.054	21.068	21.06	0.05%
3.00E+03	3.48	24.385	24.561	24.47	0.51%
3.00E+02	2.48	27.896	27.939	27.92	0.11%
3.00E+01	1.48	30.970	31.011	30.99	0.09%
3.00	0.48	34.534	34.461	34.50	0.15%
Amplification efficiency				99.57%

Residual DNA fragment(≥ 517bp) Detection

Standard		Target gene copy number standard curve (FAM)
Concentration (fg/μL) \| Log10 Concentration		Ct Value 1	Ct Value 2	Ct Value 3	CV%
3.00E+05	5.48	17.707	17.800	17.75	0.37%
3.00E+04	4.48	21.054	21.068	21.06	0.05%
3.00E+03	3.48	24.385	24.561	24.47	0.51%
3.00E+02	2.48	27.896	27.939	27.92	0.11%
3.00E+01	1.48	30.970	31.011	30.99	0.09%
3.00	0.48	34.534	34.461	34.50	0.15%
Amplification efficiency				93.72%