A CD19 CAR-NK vector (pHi-HV01-EF1α-CD19 CAR) with a specific killing function and convenient identification of infection efficiency has been developed by Jiangsu Hillgene based on the second-generation CAR-NK technology. The vector is added with the costimulatory domain, 4-1BB, which prolongs the survival time of CAR-NK cells and promotes the proliferation of cells, and the kanamycin resistance is also modified so that it can meet the requirements of domestic and foreign regulations and policies.
| Specification | 0.5ml/strip |
| Validity period | 24 months |
| Storage conditions | -80℃ |
| LOQ | NA |
| LOD | NA |
| IND Filing | NA |
| FDA Filing | NA |
CD19 CAR-NK lentiviral packaging is performed using Jiangsu Hillgene’s BaEV envelope four-plasmid system. BaEV pseudotyped LVVs can efficiently transduct NK cells and improve the infection efficiency of NK cells, and CD19 CAR-NK can efficiently and specifically kill CD19-positive cells, which provides a very good research tool for preclinical studies of gene/cell therapy and is of great significance for promoting the development of gene/cell therapy.
CD19 CAR-NK Vector Design
CD19 CAR-NK Lentivirus Preparation Process
CD19 CAR-NK Lentivirus Quality Standard
| Description | Detection items | Detection Method | Quality Standard | Result |
| Harvest liquid | HCP | ELISA | Report the result | 10334.75ng/mL |
| DS/finished products | p24 | ELISA | Report the result | 69171.84/mL |
| TU | FACS(293T) | Report the result | 2.38E+07 TU/mL |
*Specific products to be sold are determined according to the actual batch.
1. CD19 CAR-NK Cell Preparation Process
2. CAR-NK Detection Data
3. CAR-NK Cell Quality Standard
| Detection items | Detection Method | Quality Standard | Result |
| CAR positive rate | FACS | ≥10.0% | 40.98% |
| CD3-CD56+ | FACS | Report the result | 99.57% |
| CD16+ | FACS | Report the result | 90.81% |
| NKG2D | FACS | Report the result | 94.10% |
| Mycoplasma | qPCR | Shall meet the requirement | Negative |
| Sterility | Sterility rapid test | Shall meet the requirement | Negative |
| Product name | Cat.No. | specification |
| CD19 CAR-NK Premade Lentivirus | HG-CN1901 | 0.5mL |
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info@hillgene.com 
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