Pichia Pastoris Hcp Elisa Assay Kit

This product utilizes a double-antibody sandwich method to detect residual Pichia pastoris host cell proteins (HCP) in samples. A microtiter plate pre-coated with  capture antibody specific to Pichia pastoris HCP is used. After adding samples and standards, the capture antibody specifically binds to the Pichia pastoris HCP. Following a wash step to remove impurities, an enzyme-labeled antibody is added. After another wash step to remove unbound enzyme-labeled antibodies, a substrate is added for color development. The reaction is then terminated, and the absorbance is read. The absorbance value of the sample is directly proportional to the amount of Pichia pastoris HCP detected in the sample. By comparing the absorbance to a standard curve and multiplying by the corresponding dilution factor, the residual amount of Pichia pastoris HCP in the sample can be determined.